A highly sensitive, simple, and rapid liquid chromatography tandem mass spectrometry method to simultaneously determine\nblonanserin and blonanserin C in human plasma with AD-5332 as internal standard (IS) was established. A simple direct protein\nprecipitation method was used for the sample pretreatment, and chromatographic separation was performed on aWaters XBridge\nC8 (4.6 Ã?â?? 150 mm, 3.5 ????m) column. The mobile phase consists of a mixture of 10mM ammonium formate and 0.1% formic acid\nin water (A) and 0.1% formic acid in methanol (B). To quantify blonanserin, blonanserin C, and IS, multiple reaction monitoring\n(MRM) was performed in positive ESI mode. The calibration curve was linear in the concentration range of 0.012ââ?¬â??5.78 ng?mL?1\nfor blonanserin and 0.023ââ?¬â??11.57 ng?mL?1 for blonanserin C (????2 > 0.9990). The intra- and interday precision of three quality control\n(QC) levels in plasma were less than 7.5%. Finally, the current simple, sensitive, and accurate LC-MS/MS method was successfully\napplied to investigate the pharmacokinetics of blonanserin and blonanserin C in healthy Chinese volunteers.
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